https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Proteomic analysis reveals that topoisomerase 2A is associated with defective sperm head morphology https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46784 Wed 30 Nov 2022 13:21:59 AEDT ]]> DNA variants are an unlikely explanation for the changing quality of spermatozoa within the same individual https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43721 Wed 28 Sep 2022 10:29:52 AEST ]]> Post-ejaculatory changes in the metabolic status of rat spermatozoa as measured by GC-MS https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19948 Wed 27 Jul 2022 13:58:56 AEST ]]> Quantitative glycopeptide changes in rat sperm during epididymal transit https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30252 N-linked sialylated glycoproteins in rat epididymal sperm and investigate whether they are regulated during epididymal transit. Sialylated glycopeptides from caput, corpus, and cauda spermatozoa were enriched using titanium dioxide beads. Bound N-linked glycopeptides were released by enzymatic deglycosylation using PNGase F and then analyzed by liquid chromatography tandem-mass spectrometry. A total of 92 unique N-linked sialylated glycopeptides were identified from 65 different proteins. These included members of the disintegrin and metalloproteinase domain-containing protein family (ADAM), Basigin, and Testis-expressed protein 101 (TEX101). Remarkably, label-free quantification showed that more than half of these peptides (48/92) were regulated during epididymal transit. Of interest, the protein TEX101 exhibited PNGase F-resistant deglycosylation under the conditions used in this study. The results from this study showed that changes in the N-linked sialoglycoprotein profile is a major hallmark of sperm maturation in rats.]]> Wed 11 Apr 2018 17:21:10 AEST ]]> The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15122 Wed 11 Apr 2018 15:13:29 AEST ]]> Analysis of the mechanism by which calcium negatively regulates the tyrosine phoshorylation cascade associated with sperm capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2527 Wed 11 Apr 2018 14:30:38 AEST ]]> Deficiency in outer dense fiber 1 is a marker and potential driver of idiopathic male infertility https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25775 Wed 11 Apr 2018 13:20:33 AEST ]]> Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1286 Wed 11 Apr 2018 11:40:47 AEST ]]> Mass Spectrometry Reveals New Insights into the Production of Superoxide Anions and 4-Hydroxynonenal Adducted Proteins in Human Sperm https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41655 Wed 10 Aug 2022 10:07:33 AEST ]]> Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7 Thu 25 Jul 2013 09:10:12 AEST ]]> Proteomic analysis of good- and poor-quality human sperm demonstrates that several proteins are routinely aberrantly regulated https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41254 Sat 30 Jul 2022 12:54:22 AEST ]]> Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa: its implications during capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7891 Sat 24 Mar 2018 08:35:08 AEDT ]]> Label-free quantitation of phosphopeptide changes during rat sperm capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9963 Sat 24 Mar 2018 08:14:26 AEDT ]]> Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10935 Sat 24 Mar 2018 08:13:21 AEDT ]]> Phosphopeptide analysis of rodent epididymal spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19261 Sat 24 Mar 2018 08:06:38 AEDT ]]> Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17528 Sat 24 Mar 2018 08:03:55 AEDT ]]> Head and flagella subcompartmental proteomic analysis of human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20019 Sat 24 Mar 2018 07:50:53 AEDT ]]> The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5181 Sat 24 Mar 2018 07:47:49 AEDT ]]> The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5289 Sat 24 Mar 2018 07:46:30 AEDT ]]> Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41 Sat 24 Mar 2018 07:42:12 AEDT ]]> Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25432 Sat 24 Mar 2018 07:31:54 AEDT ]]> Analysis of protein thiol changes occurring during rat sperm epididymal maturation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27256 Sat 24 Mar 2018 07:29:12 AEDT ]]> Defining the mechanisms by which the reactive oxygen species by-product, 4-hydroxynonenal, affects human sperm cell function https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27253 Sat 24 Mar 2018 07:29:09 AEDT ]]>